Application of reverse genetic systems to study Beet soil-borne mosaic virus and Beet necrotic yellow vein virus molecular biology, the interaction of species and their use as biotechnological tool

Lingua: inglese

Editore: Cuvillier, Cuvillier Jun 2018, 2018

3736998147 / 9783736998148

Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germaniabuchversandmimpf2000

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Venditore AbeBooks dal 23 gennaio 2017

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This item is printed on demand - Print on Demand Titel. Neuware -Two members of the Benyviridae family, Beet soil borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organisation, host range and high sequence similarity. To understand functional differences in molecular biology, pathogenicity mechanisms, symptom expression as well as interaction with the host and between viral species, a reverse genetic system represents a prerequisite. Infectious cDNA full length clones of both viruses were constructed by isothermal in vitro recombination. Artificial formation of BNYVV and BSBMV RNA1+2 reassortants were viable and spread long distance in N. benthamiana. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. Moreover, fluorescence labelled full length clones were achieved by replacing a part of the RNA2 encoded coat protein read through domain. Co infection experiments with labelled BSBMV and BNYVV showed that both viruses remained spatially separated after N. benthamiana agroinoculation. In contrast, a mixture of BSBMV with an unrelated virus resulted in no co infection exclusion. In super infection experiments, BSBMV and BNYVV were unable to establish a secondary infection in plants that were previously infected with BNYVV or BSBMV. In addition, a virus induced gene silencing (VIGS) system was developed. Both viruses were equipped with fragments of the magnesium chelatase subunit H and phytoene desaturase genes. Silencing phenotypes in N. benthamiana induced by both target genes, were comparable to those described in the literature.Cuvillier Verlag, Nonnenstieg 8, 37075 Göttingen 154 pp. Englisch.…

Codice articolo 9783736998148

Dati bibliografici

Titolo
Application of reverse genetic systems to study Beet soil-borne mosaic virus and Beet necrotic yellow vein virus molecular biology, the interaction of species and their use as biotechnological tool
Autore
Marlene Laufer
Editore
Cuvillier, Cuvillier Jun 2018
Anno di pubblicazione
2018
Condizione
Neu
Rilegatura
Taschenbuch
Lingua
inglese
ISBN 10
3736998147
ISBN 13
9783736998148
Peso dell'articolo
209 grammi
Dimensioni
210x148x9 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germania

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Venditore AbeBooks dal 23 gennaio 2017

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