Bacterial Efflux Systems (Volume 723) (Methods in Enzymology, Volume 723). Questo articolo non è disponibile.Bacterial Efflux Systems (Volume 723) (Methods in Enzymology, Volume 723)

The volume covers experimental and computational approaches to study the function, structure and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses, and in vivo activity analysis. Essays for evaluating activity are described, including reconstitution of system for quantitative analysis of efflux activity. The topics include sample preparation and analysis for structure determination of pump components and assemblies and with bound ligands and inhibitors, by x-ray crystallography and electron cryomicroscopy. Methods for in situ structural analysis by electron tomography are also described. Topics include partners of the efflux systems and the biogenesis of the nanomachines.

Ben Luisi works in the Department of Biochemistry at the University of Cambridge, where he has an appointment as professor of structural biology. He and his colleagues study the structure and mechanism of efflux pumps and the riboregulatory machinery that help to control the expression of the pumps and other complex systems in bacteria.