Titolo: Bacterial Secretion Systems: Methods and Protocols (Methods in Molecular Biology, 2715)
Editore: Humana
Data di pubblicazione: 2023
Lingua: Inglese
ISBN 10: 1071634445
ISBN 13: 9781071634448
Rilegatura: Rilegato
Edizione: seconda edizione
Condizione: New

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Riassunto

This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

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