Basic Cloning Procedures

Berzins Valdis

ISBN 10: 364248977X / ISBN 13: 9783642489778

Editore: Springer, 2012

PRINT ON DEMAND pp. 180. Codice articolo 1898175047

Riassunto:

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.

Dalla quarta di copertina: This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Dati bibliografici

Titolo: Basic Cloning Procedures
Casa editrice: Springer
Data di pubblicazione: 2012
Legatura: Brossura
Condizione: New