A Beginner's Guide to Microarrays

Eric M. Blalock

ISBN 10: 1402074727 / ISBN 13: 9781402074721

Editore: Springer US, Springer US Jul 2003, 2003

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Neuware -A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:Cleaning and coating glass slidesDesigning oligonucleotide probesConstructing arrays for the detection and quantification of different bacterial speciesPreparing spotting solutionsTroubleshooting spotting problemsSetting up and running a core facilityNormalizing background signal and controlling for systematic varianceDesigning experiments for maximum effectAnalyzing data with statistical proceduresClustering data with machine-learning protocols. 368 pp. Englisch.

Codice articolo 9781402074721

Dati bibliografici

• Titolo: A Beginner's Guide to Microarrays
• Editore: Springer US, Springer US Jul 2003
• Data di pubblicazione: 2003
• Lingua: Inglese
• ISBN 10: 1402074727
• ISBN 13: 9781402074721
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 241 L x 160 A x 28 P (millimetri) 241 millimetri di larghezza per 160 millimetri di altezza per 28 millimetri di profondità
• Peso: 784 grammi

Descrizione articolo

Riassunto

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:

-Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols. 

Recensione

From the reviews:

"The book offers a broad coverage of many of the most important aspects of microarray technology ... . Despite the wide field broached by this book, one of its chief successes lies with the fact that in each chapter the authors provide a clear review of every step of the technique, including both its potential and pitfalls. This allows all readers, especially beginners, to understand the development and impact of this important technique. ... Readers will find this beginner’s guide book very informative ... ." (Guillermina Lopez-Bendito, Journal of Chemical Neuroanatomy, Vol. 30, 2005)