Bioconjugation Protocols : Stragegies and Methods

Mark, Sonny S. (EDT)

ISBN 10: 1617791504 / ISBN 13: 9781617791505

Editore: Humana, 2011

Codice articolo 12536374-n

Riassunto:

<p>Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology.  In this second edition of <i>Bioconjugation Protocols:  Strategies and Methods</i>, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering.  These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches.  Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices).  All the protocols follow the successful <i>Methods in Molecular Biology</i>^TM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.</p><p> </p>Cutting-edge and highly practical, <i>Bioconjugation Protocols: Strategies and Methods, Second Edition</i> offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Dalla quarta di copertina:

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular Biology^TM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a widevariety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Dati bibliografici

Titolo: Bioconjugation Protocols : Stragegies and ...
Casa editrice: Humana
Data di pubblicazione: 2011
Legatura: Rilegato
Condizione: New
Edizione: seconda edizione