CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY | FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY
Jiaqian Wu
ISBN 10: 3844320601 ISBN 13: 9783844320602
Editore: LAP LAMBERT Academic Publishing, 2011
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CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY | FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY | Jiaqian Wu | Taschenbuch | 204 S. | Englisch | 2011 | LAP LAMBERT Academic Publishing | EAN 9783844320602 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Codice articolo 107018860
Riassunto:
In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.
L'autore: Jiaqian Wu, Ph.D. authored many peer-reviewed publications in the field of Genetics, Genomics and Stem Cell research. She won multiple awards and has one patent. Jiaqian has given talks at international conferences and research institutes. Jiaqian is currently an assistant professor at the University of Texas Medical School at Houston.
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Titolo: CHARACTERIZE MAMMALIAN TRANSCRIPTOME ...
Casa editrice: LAP LAMBERT Academic Publishing
Data di pubblicazione: 2011
Legatura: Taschenbuch
Condizione: Neu