CRISPR-Cas Methods

M. Tofazzal Islam

ISBN 10: 1071606158 / ISBN 13: 9781071606155

Editore: Springer US Jun 2020, 2020

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This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism. 296 pp. Englisch.

Codice articolo 9781071606155

Dati bibliografici

• Titolo: CRISPR-Cas Methods
• Editore: Springer US Jun 2020
• Data di pubblicazione: 2020
• Lingua: Inglese
• ISBN 10: 1071606158
• ISBN 13: 9781071606155
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 260 L x 183 A x 22 P (millimetri) 260 millimetri di larghezza per 183 millimetri di altezza per 22 millimetri di profondità
• Peso: 758 grammi

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Riassunto

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 

Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

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