Capillary Electrophoresis Of Nucleic Acids: Introduction To The Capillary Electrophoresis Of Nucleic Acids

ISBN 10: 0896037797 / ISBN 13: 9780896037793

Editore: Humana, 2000

Brand New Original US Edition. Customer service! Satisfaction Guaranteed. Codice articolo ASNT3-62201

Riassunto:

Capillary Electrophoresis (CE) provides researchers with the ability to separate and analyze very small amounts of DNA. CE offers important advantages over other available methods because of the speed and reproducibility of analysis. Further, the high sensitivity of detection and the ability to increase sample throughput with parallel analysis has led to the creation of a full range of techniques for the analysis of DNA molecules, from modified DNA-adducts and single strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids provides these analytical protocols, which can be used for detection and analysis of mutations and modifications to precise DNA loci through to entire genomes of organisms. Volume I broadly addresses instrumentation, signal detection and the capillary environment, as well as the integration of mass spectrometry and CE for the analysis of small oligonucleotides and modified nucleotides. It opens with a description of the basic CE instrumentation and discusses DNA sequencing by CE and the methods for the manufacture of microchip devices. Also covered are issues regarding the choices of separation media, and the fast separations of large DNA molecules and chromosomes using pulsed-field capillary electrophoresis. The book closes by offering choices for the analysis of small therapeutic oligonucleotides, nucleosides, ribonucleotides and DNA metabolism product by CE as well as those products arising from environmental and cellular damage through disease.

Dalla quarta di copertina: The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic Acids, covers the practical and theoretical considerations behind the use of capillary electrophoresis for the analysis of small oligonucleotides and modified nucleotides. Along with detailed instructions ensuring ready reproducibility, these protocols offer time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides by CE. The companion volume, Practical Applications of Capillary Electrophoresis, addresses techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.

Dati bibliografici

Titolo: Capillary Electrophoresis Of Nucleic Acids: ...
Casa editrice: Humana
Data di pubblicazione: 2000
Legatura: Rilegato
Condizione: New