Cell Separation : Fundamentals, Analytical and Preparative Methods

Ashok Kumar

ISBN 10: 3540752625 / ISBN 13: 9783540752622

Editore: Springer Berlin Heidelberg, 2007

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Druck auf Anfrage Neuware - Printed after ordering - Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci c and selective techniques have emerged as the demand for isolating a speci c cell type for various biological applications has increased. Ef cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci c separation of a discrete population of cells from a mixture. For example, in the eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells,regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro ltration, ultra ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci cityandproblemswithscalingup.

Codice articolo 9783540752622

Dati bibliografici

• Titolo: Cell Separation : Fundamentals, Analytical and Preparative Methods
• Editore: Springer Berlin Heidelberg
• Data di pubblicazione: 2007
• Lingua: Inglese
• ISBN 10: 3540752625
• ISBN 13: 9783540752622
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 241 L x 160 A x 18 P (millimetri) 241 millimetri di larghezza per 160 millimetri di altezza per 18 millimetri di profondità
• Peso: 500 grammi

Descrizione articolo

Riassunto

micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup.

Contenuti

M. B. Dainiak, A. Kumar, I.Y. Galaev, B. Mattiasson: Methods in Cell Separations.- S.F. Ibrahim, G. van den Engh: Flow Cytometry and Cell Sorting.- A.A. Neurauter, M. Bonyhadi, E. Lien, L. Nøkleby, E. Ruud, S. Camacho, T. Aarvak: Cell Isolation and Expansion Using Dynabeads®.- J. Hubble: Affinity Adsorption of Cells to Surfaces and Strategies for Cell Detachment.- M.B. Dainiak, I.Y. Galaev, A. Kumar, F.M. Plieva, B. Mattiasson: Chromatography of Living Cells Using Supermacroporous Hydrogels, Cryogels.- R.E. Nordon, S. Craig: Hollow-Fibre Affinity Cell Separation.- J.M.S. Cabral: Cell Partitioning in Aqueous Two-Phase Polymer Systems.- M. Kamihira, A. Kumar: Development of Separation Technique for Stem Cells.-