The state-of-the-art methods collected here are designed to analyze the relationship between chromatin structure and function, and to elucidate the molecular mechanisms that control such vital cellular functions as transcription, replication, recombination, and DNA repair. These proven methods cover a wide range of topics, from powerful cell-free systems for the reconstitution of chromatin heterogeneity in vitro, to both classical and cutting-edge techniques for in vivo analysis of protein-DNA interactions. Each method includes detailed step-by-step instructions to ensure successful replication along with helpful notes about how to avoid pitfalls. All authors are leading scientists, well known for their methodological expertise in the chromatin research.

Recensione

"Laboratories working with chromatin or concerned with mammalian gene expression in context will profit from the focus in this volume."-Doody's Health Science Book Review Journal

"Chromatin Protocols represents an exhaustive collection (over 500 pages) of back-ground information and protocols to investigate various aspects of chromatin structure and function....The breadth of the topics covered makes Chromatin Protocols appealing to most cell biologists and a must for specialists in the field....the range and richness of the protocols treated make this volume a valuable reference...Your lab should definitely get it and your library should get it, as the book will certainly remain up to date in the years to come."-Cell Biology International

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