Comprehensive Virology (Paperback)

H. Fraenkel-Conrat

ISBN 10: 1468427148 / ISBN 13: 9781468427141

Editore: Springer-Verlag New York Inc., New York, NY, 2012

Paperback. The time seems ripe for a critical compendium of that segment of the biological universe we call viruses. Virology, as a science, having passed only recently through its descriptive phase of naming and num bering, has probably reached that stage at which relatively few new~ truly new~viruses will be discovered. Triggered by the intellectual probes and techniques of molecular biology, genetics, biochemical cytology, and high-resolution microscopy and spectroscopy, the field has experienced a genuine information explosion. Few serious attempts have been made to chronicle these events. This comprehensive series, which will comprise some 6000 pages in a total of about 22 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. In this way, we hope to place the entire field in perspective, and to develop an invalu able reference and sourcebook for researchers and students at all levels. This series is designed as a continuum that can be entered anywhere, but which also provides a logical progression of developing facts and integrated concepts. This comprehensive series, which will comprise some 6000 pages in a total of about 22 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Codice articolo 9781468427141

Riassunto:

The time seems ripe for a critical compendium of that segment of the biological universe we call viruses. Virology, as a science, having passed only recently through its descriptive phase of naming and num­ bering, has probably reached that stage at which relatively few new~ truly new~viruses will be discovered. Triggered by the intellectual probes and techniques of molecular biology, genetics, biochemical cytology, and high-resolution microscopy and spectroscopy, the field has experienced a genuine information explosion. Few serious attempts have been made to chronicle these events. This comprehensive series, which will comprise some 6000 pages in a total of about 22 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. In this way, we hope to place the entire field in perspective, and to develop an invalu­ able reference and sourcebook for researchers and students at all levels. This series is designed as a continuum that can be entered anywhere, but which also provides a logical progression of developing facts and integrated concepts.

Contenuti: 1 The Isometric Single-Stranded DNA Phages.- 1. Introduction.- 1.1. A Circular, Single-Stranded DNA Molecule within a Tailless Capsid.- 1.2. Genes and Proteins of the Isometric Phages.- 1.3. Enzymatic and Chemical Studies.- 1.4. Properties of øX174 Virus and øX174 Viral DNA Forms.- 2. Formation of the Parental RF.- 2.1. Adsorption to the Cell.- 2.2. Eclipse of the Virion.- 2.3. Synthesis of a Complementary Strand.- 3. Expression of the Viral Genome.- 3.1. Transcription.- 3.2. Translation.- 3.3. Superinfection Exclusion and Inhibition of Host DNA Synthesis.- 4. RF Replication.- 4.1. Function of Gene A.- 4.2. The rep Mutant and Other Required Host Cell Functions.- 4.3. Origin of øX174 RF Replication.- 4.4. Nascent RF Molecules Contain Gaps.- 4.5. Structure of the Replicating Intermediate: The “Reciprocating Strand” Model.- 4.6. Selectivity of Subsequent Replication.- 4.7. Radiobiological Experiments and Parent-to-Progeny Transfer.- 4.8. Role of the Membrane.- 5. Single-Stranded DNA Synthesis.- 5.1. Asymmetric Displacement Replication.- 5.2. Methylation.- 5.3. Circularization.- 5.4. Viral Proteins Required for Single-Stranded DNA Synthesis.- 6. Biogenesis of the Mature Virion.- 6.1. Assembly of the Virus Particle.- 6.2. Lysis of the Cell.- 7. Recombination.- 7.1. Structural Intermediates.- 7.2. Gene Functions Involved.- 7.3. Heteroduplex Repair and Single-Strand Aggression.- 8. References.- 2 Replication of Filamentous Bacteriophages.- 1. Introduction.- 2. Structure of the Ff Virion.- 3. Structure of Ff DNA.- 3.1. Single-Stranded Ring Structure of Ff DNA.- 3.2. Conformations of Ff DNA.- 3.3. Self-Complementary Regions in Ff DNA.- 3.4. Pyrimidine Tracts in Ff DNA.- 3.5. RNA Polymerase Binding Sites.- 3.6. DNA Sequence Studies.- 3.7. E. coli B-Specific Modification and Restriction Sites.- 3.8. Type II Restriction Sites.- 3.9. Miniature Forms of Ff DNA.- 3.10. Multiple-Length Forms of Ff DNA.- 4. Phage Attachment and Initiation of Infection.- 5. Parental RF Formation (SS?RF).- 5.1. Involvement of a Minor Capsid Protein.- 5.2. Drug Sensitivity of Parental RF Formation.- 5.3. Parental RF Formation by Bacterial Enzymes.- 5.4. A Unique Gap in the Parental Complementary Strand of M13 DNA.- 5.5. Location of the RNA Primer at the 5’-Terminus of the Complementary Strand.- 6. RF Replication (RF?RF).- 6.1. Requirement for a Phage Function.- 6.2. Involvement of Host Functions.- 6.3. Mechanics of Ff RF Replication.- 6.4. Membrane Attachment of Replicating RF.- 6.5. Function of the RF Pool.- 7. Single-Strand Synthesis (RF?SS).- 7.1. Kinetics of Single-Strand Synthesis.- 7.2. Requirement for Gene 5 and Gene 2 Proteins in Single-Strand Synthesis.- 7.3. Association of Gene 5 Protein with Viral Single Strands.- 7.4. Requirement for Host Functions in Single-Strand Synthesis.- 7.5. Mechanics of Single-Strand Synthesis.- 8. Expression of the Ff Genome.- 8.1. Regulated Synthesis of Gene Products.- 8.2. Transcription of the Ff Genome.- 8.3. Translation of Ff Gene Transcripts.- 9. Future Areas of Research.- 10. References.- 3 Reproduction of Large Virulent Bacteriophages.- 1. Introduction.- 1.1. Overview of the Field.- 1.2. Scope of This Chapter.- 2. Structural Features of Large Virulent Phages.- 3. Reproduction of T-Even Coliphages.- 3.1. Overview of the Phage Life Cycle.- 3.2. Physiological Genetics of T-Even Coliphages.- 3.3. Early Steps in Infection.- 3.4. Physiological Roles of T-Even Phage-Coded Enzymes.- 3.5. DNA Replication.- 3.6. Control of Gene Expression.- 3.7. Late Functions.- 4. Reproduction of T-Odd Coliphages.- 4.1. T1.- 4.2. T3 and T7.- 4.3. T5.- 5. Bacillus subtilis Phages.- 5.1. Phages with Hydroxymethyluracil-Containing DNA.- 5.2. Phages with Uracil-Containing DNA.- 5.3. Small B. subtilis Phages.- 5.4. Other B. subtilis Phages.- 6. Other Phages.- 7. References.

Dati bibliografici

Titolo: Comprehensive Virology (Paperback)
Casa editrice: Springer-Verlag New York Inc., New York, NY
Data di pubblicazione: 2012
Legatura: Paperback
Condizione: new