Craniofacial Development | Methods and Protocols

Sebastian Dworkin

ISBN 10: 1071618466 / ISBN 13: 9781071618462

Editore: Springer US, 2021

Craniofacial Development | Methods and Protocols | Sebastian Dworkin | Buch | xii | Englisch | 2021 | Springer US | EAN 9781071618462 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Codice articolo 120202354

Riassunto:

<p>This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (<i>c. elegans</i>, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and&nbsp;<i>in vivo</i>&nbsp;cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using&nbsp;<i>in-situ&nbsp;</i>hybridisation and&nbsp;single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as&nbsp;CRISPR/Cas9-mediated deletion.&nbsp;Written in the format of the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.&nbsp;</p>Authoritative and cutting-edge,&nbsp;<i>Craniofacial Development: Methods and Protocols</i>&nbsp;aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.&nbsp;<p></p><div><br></div>

Dalla quarta di copertina: This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (<i>c. elegans</i>, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and&nbsp;<i>in vivo</i>&nbsp;cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using&nbsp;<i>in-situ&nbsp;</i>hybridisation and&nbsp;single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as&nbsp;CRISPR/Cas9-mediated deletion.&nbsp;Written in the format of the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.&nbsp;<div><br>Authoritative and cutting-edge,&nbsp;<i>Craniofacial Development: Methods and Protocols</i>&nbsp;aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.&nbsp;<p></p><div><br></div></div>

Dati bibliografici

Titolo: Craniofacial Development | Methods and ...
Casa editrice: Springer US
Data di pubblicazione: 2021
Legatura: Buch
Condizione: Neu