In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.
With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
1. BioBrick Assembly Standards and Techniques and Associated Software Tools
Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås, and Rahmi Lale
2. Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning
Ryan E. Hill, Julian J. Eaton-Rye
3. Quick and Clean Cloning
Frank Thieme and Sylvestre Marillonnet
4. Hierarchical Ligation-Independent Assembly of PCR Fragments
Jonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson
5. USER-Derived Cloning Methods and Their Primer Design
Bo Salomonsen, Uffe H Mortensen, Barbara A Halkier
6. Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly
Yoav Peleg and Tamar Unger
7. A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform
Ariel Erijman, Julia M. Shifman, and Yoav Peleg
8. Circular Polymerase Extension Cloning
Jiayuan Quan and Jingdong Tian
9. Golden Gate Cloning
Carola Engler and Sylvestre Marillonnet
10. Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell, Diego Orzaez
11. FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
Eric R. Geertsma
12. Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing
Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada
13. Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
Chun You and Y.-H. Percival Zhang
14. Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter, and Dominic Esposito
15. Application of In-Fusion™ Cloning for the Parallel Construction of Escherichia coli Expression Vectors
Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert, Raymond J. Owens
16. SeamLess Ligation Cloning Extract(SLiCE)Cloning Method
Yongwei Zhang, Uwe Werling, and Winfried Edelmann
17. j5 DNA Assembly Design Automation
Nathan J. Hillson
18. FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis
Ruslan Kalendar, David Lee, and Alan H. Schulman
