This is an ex-library book and may have the usual library/used-book markings inside.This book has soft covers. In fair condition, suitable as a study copy. Please note the Image in this listing is a stock photo and may not match the covers of the actual item,700grams, ISBN:9780199634804.

Codice articolo 9230925

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Dati bibliografici

Titolo

DNA Cloning 4: A Practical Approach - Mammalian Systems

Editore

IRL Press

Data di pubblicazione

1995

Lingua

Inglese

ISBN 10

0199634807

ISBN 13

9780199634804

Rilegatura

Brossura

Edizione

seconda edizione

Condizione

Fair

Peso

700 grammi

Cataloghi dei venditori

Biology

Descrizione articolo

Riassunto

DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments.

This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.

Contenuti

1.: High efficiency gene transfer into mammalian cells
2.: Construction and characterization of Vaccinia virus recombinants
3.: Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
4.: Retroviral vectors
5.: Genetic manipulation of embryonic stem cells
6.: Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs
7.: Genomic and expression analysis of transgenic animals
8.: Expression using a defective Herpes simplex virus (HSV-1) vector system
9.: Adenovirus vectors

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Descrizione libreria

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