Former library book. Solid binding. Minimal shelfwear. Please note the image in this listing is a stock photo and may not match the covers of the actual item.

Codice articolo 123478426

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Dati bibliografici

Titolo: DNA Cloning: A Practical Approach Volume 4: Mammalian Systems
Editore: Oxford University Press
Data di pubblicazione: 1996
Lingua: Inglese
ISBN 10: 0199634807
ISBN 13: 9780199634804
Rilegatura: Softcover
Edizione: seconda edizione
Condizione: Good
Condizione della sovraccoperta: No Jacket
Tipo di libro: Ex-Library

Descrizione articolo

Riassunto

DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments.

This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.

Contenuti

1.: High efficiency gene transfer into mammalian cells
2.: Construction and characterization of Vaccinia virus recombinants
3.: Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
4.: Retroviral vectors
5.: Genetic manipulation of embryonic stem cells
6.: Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs
7.: Genomic and expression analysis of transgenic animals
8.: Expression using a defective Herpes simplex virus (HSV-1) vector system
9.: Adenovirus vectors

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