Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater

Lingua: inglese

Editore: IWA Publishing Aug 2004, 2004

1843397226 / 9781843397229

Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH

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Neuware - Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method.

Codice articolo 9781843397229

Titolo
Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater
Autore
T. Cromeans
Editore
IWA Publishing Aug 2004
Anno di pubblicazione
2004
Condizione
Neu
Rilegatura
Taschenbuch
Lingua
inglese
ISBN 10
1843397226
ISBN 13
9781843397229
Peso dell'articolo
224 grammi
Dimensioni
279x211x9 mm

AHA-BUCH GmbH

Einbeck, Germania

Venditore con 5 stelle

Venditore AbeBooks dal 14 agosto 2006

Tariffe di spedizione da Germania a U.S.A.

ArticoloDa 30 a 40 giorni lavorativiDa 7 a 14 giorni lavorativi
Primo articoloEUR 61,12EUR 71,12
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