Development and Validation of Indicators for the Production and Quality of Seed Cultures

Lingua: inglese

Editore: Cuvillier

3869558474 / 9783869558479

Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH

Venditore con 5 stelle

Venditore AbeBooks dal 14 agosto 2006

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Descrizione dell’articolo da parte del venditore

nach der Bestellung gedruckt Neuware - Printed after ordering - Cultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. This work hence introduces a total of nine spore quality indicators and subsequently correlates the sporulation environome to the properties of the generated spores and their performance in submerged cultivation. The model organism is Aspergillus ochraceus while the model process is the hydroxylation of a steroid. A positive correlation between the spore yield and the substrate's carbon content on solid medium was found with oat bran agar giving best yields of 26 107 spores/mL at carbon concentrations of 15.4 g/L in the solid medium. The assessment of the conidial metabolome revealed the presence of 126 metabolites. Particularly polyols, such as glycerol, erythritol and ribitol, are found at high concentrations of up to 55 ng/105 spores. It was further shown, that several of these metabolites are degraded within the spores during storage for 7 d in saline solution. Their concentrations also strongly correlate with the storage resistance of the conidia as well as with their performance in subsequent submerged cultivations. Highest reactant turnover of approx. 80 % was determined after 72 h of cultivation with spores generated on wheat bran agar. The culture performance is further a function of the portion of protein bound NADH, which was determined and spatially visualized by means of two-photon fluorescence lifetime imaging.…

Codice articolo 9783869558479

Dati bibliografici

Titolo
Development and Validation of Indicators for the Production and Quality of Seed Cultures
Autore
Matthias Gehder
Editore
Cuvillier
Condizione
Neu
Rilegatura
Taschenbuch
Lingua
inglese
ISBN 10
3869558474
ISBN 13
9783869558479
Peso dell'articolo
244 grammi
Dimensioni
210x148x11 mm

AHA-BUCH GmbH

Einbeck, Germania

Venditore con 5 stelle

Venditore AbeBooks dal 14 agosto 2006

Tariffe di spedizione da Germania a U.S.A.

ArticoloDa 7 a 10 giorni lavorativiDa 5 a 7 giorni lavorativi
Primo articoloEUR 35,00EUR 45,00
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