Digital PCR | Methods and Protocols

Guillaume Pavlovic

ISBN 10: 1071647660 / ISBN 13: 9781071647660

Editore: Humana, 2025

Digital PCR | Methods and Protocols | Guillaume Pavlovic | Buch | xi | Englisch | 2025 | Humana | EAN 9781071647660 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Codice articolo 134132040

Riassunto:

This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Digital PCR: Methods and Protocols serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision.

Dalla quarta di copertina:

This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Digital PCR: Methods and Protocols serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision.

Dati bibliografici

Titolo: Digital PCR | Methods and Protocols
Casa editrice: Humana
Data di pubblicazione: 2025
Legatura: Buch
Condizione: Neu