Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology, 2461)

Currin, Andrew (Editor) / Swainston, Neil (Editor)

ISBN 10: 1071621548 / ISBN 13: 9781071621547

Editore: Humana, 2023

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289 pages. 10.00x7.01x0.62 inches. In Stock.

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Dati bibliografici

• Titolo: Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology, 2461)
• Editore: Humana
• Data di pubblicazione: 2023
• Lingua: Inglese
• ISBN 10: 1071621548
• ISBN 13: 9781071621547
• Rilegatura: Paperback
• Condizione: Brand New
• Peso: 0,56 chilogrammi

Descrizione articolo

Riassunto

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.