Double-Stranded RNA : Methods and Protocols

Guanwei Wu

ISBN 10: 1071637045 / ISBN 13: 9781071637043

Editore: Springer US, Springer New York, 2025

Riguardo questo articolo

Druck auf Anfrage Neuware - Printed after ordering - This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA.

Codice articolo 9781071637043

Dati bibliografici

• Titolo: Double-Stranded RNA : Methods and Protocols
• Editore: Springer US, Springer New York
• Data di pubblicazione: 2025
• Lingua: Inglese
• ISBN 10: 1071637045
• ISBN 13: 9781071637043
• Rilegatura: Taschenbuch
• Condizione: Neu
• Dimensioni: 254 L x 178 A x 9 P (millimetri) 254 millimetri di larghezza per 178 millimetri di altezza per 9 millimetri di profondità
• Peso: 308 grammi

Descrizione articolo

Riassunto

This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. 

Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA.