Emerging Model Organisms

Wei Wang

ISBN 10: 1071628747 / ISBN 13: 9781071628744

Editore: Springer US, Springer US, 2023

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Druck auf Anfrage Neuware - Printed after ordering - This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In theNeuromethodsseries style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Authoritative and cutting-edge,Emerging Model Organisms aims to be auseful practical guide to researches to help further their study in this field.

Codice articolo 9781071628744

Dati bibliografici

• Titolo: Emerging Model Organisms
• Editore: Springer US, Springer US
• Data di pubblicazione: 2023
• Lingua: Inglese
• ISBN 10: 1071628747
• ISBN 13: 9781071628744
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 260 L x 183 A x 26 P (millimetri) 260 millimetri di larghezza per 183 millimetri di altezza per 26 millimetri di profondità
• Peso: 903 grammi

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Riassunto

This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms,  setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging,  Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Authoritative and cutting-edge, Emerging Model Organisms aims to be a useful practical guide to researches to help further their study in this field.