Enzymatic Analysis: A Practical Guide

Passonneau, Janet V.; & Oliver H. Lowry

ISBN 10: 0896032388 / ISBN 13: 9780896032385

Editore: Humana Press, Totowa, New Jersey, 1993

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Totowa, New Jersey: Humana Press, 1993. NOT print on demand. We have only this one copy, but it is available now and ready to ship today from Henderson, Nevada. The cover is in NEAR FINE condition, with only a little minor shelfwear. NEW and unread inside. NO owner's name or bookplate. Bound in the original brown laminated boards, lettered in gold. From the publisher: "Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivityall likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.". Second printing. Hardcover. Near Fine - New condition/No dust jacket. Illus. by NOT a library discard. 8vo. x, 406pp. Great Packaging, Fast Shipping.

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Dati bibliografici

• Titolo: Enzymatic Analysis: A Practical Guide
• Editore: Humana Press, Totowa, New Jersey
• Data di pubblicazione: 1993
• Lingua: Inglese
• ISBN 10: 0896032388
• ISBN 13: 9780896032385
• Rilegatura: Hardcover
• Disegnatore: NOT a library discard
• Edizione: Second printing.
• Condizione: Near Fine - New condition
• Condizione della sovraccoperta: No dust jacket
• Cataloghi dei venditori:
  • Chemistry

Descrizione articolo

Riassunto

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity―all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

Recensione

...the authors are well recognized for their contributions to histochemistry...For individuals interested in...performing quantitative histochemical methods, this book would be a worthwhile investment.-Modern Pathology