Essentials of Single-Cell Analysis

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations.

This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. 

Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan.

Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Dr.Fan-Gang Tseng received his Ph.D. degree in mechanical engineering from UCLA,USA, in 1998. He is currently a DistinguishedProfessorin the ESS Departmentas well as at the NEMS Institute, and theDeputy Director of the Biomedical Technology Research Center at NTHU. He isalso an affiliated Research Fellow of Academia Sinica, Taiwan. He was elected asan ASME fellow in 2014. His research interests are in the fields of BioNEMS,Biosensors, Micro-Fluidics, Tissue Chips, and Fuel Cells. He holds 60 patents, authored 8 book chapters, andpublished more than220 journal papers and 360 conference papers. Dr. Tseng currently serves on theeditorial boards of Applied Sciences, Journal of Circuits and Systems,International Journal of Molecule Science, Micromachines, and Open NanomedicineJournal. He received many awards, including NationalInnovation Award (twice), Outstanding Research Award (MOST and NSC, twice), First-Class ResearchAward (NSC), Mr. Wu, Da-Yo Memorial Award (NSC), NTHU Outstanding Research Award (2005-2015), and more than twelve bestpaper/poster awards from various competitions and conferences.

Dr. Tuhin Subhra Santra receivedhis Ph.D. degree in Bio-Nano Electro Mechanical Systems (Bio-NEMS) from theInstitute of Nanoengineering and Microsystems (NEMS), National Tsing HuaUniversity (NTHU), Taiwan in 2013. Currently he is a Postdoctoral Research Associate at the California Nano System Institute (CNSI),University of California, Los Angeles (UCLA), USA. His main research areas areBio-NEMS, MEMS, Single-Cell Analysis, Bio-micro/nanoFabrication, Biomedical Microdevices, Nanomedicine and etc. Dr. Santra servesas a Guest Editor for the Journal of Micromachines, Journal of Molecules,International Journal of Molecular Science, Sensors and Transducers Journal,American Journal of Nanoresearch and Application, among others. Dr. Santra hasreceived many awards such as the NTHU Outstanding Student Award in 2011 and2013, IEEE-NEMS Best Conference Paper Award in 2014, Best Poster Award at 15thNano/Microsystem Conference in 2012, Junior ResearchFellowship from theIIT-Kharagpur in 2008, OutstandingStudent Award from the Jadavpur University in2005 and 2006, Silver Medal from the VidyasagarUniversity in 2004. Dr. Santra has published more than 15 international journalpapers, six book chapters, 31 international conference papers in his researchfield. He also has one US and one Taiwan patentpending.