Gene Function Analysis

Lingua: inglese

Editore: Humana Press, 2007

1588297349 / 9781588297341

Da: Buchpark, Trebbin, GermaniaBuchpark

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Zustand: Sehr gut | Seiten: 360 | Sprache: Englisch | Produktart: Bücher | This volume of Methods in Molecular Biology focuses on techniques to determine the function of a gene. Traditionally, the function of a gene was determined following cloning, which provided its DNA sequence and an ab- ity to modify this sequence. Experiments were performed that looked for p- notypic changes in a cell line or model organism following modifications to the sequence, knocking out of the gene, or enhancing expression of the gene. In the 1990¿s, the growing sequence databases and the BLAST algorithm provided additional power by allowing identification of genes with known function that had similar sequences and potentially similar molecular mechanisms. On the experimental side, methods, such as two-hybrid screening that could directly determine the partners of specific proteins and even the domains of interaction, came into widespread use. With the advent of high-throughput technologies following completion of the human genome project and similar projects in model organisms, the n- ber of genes of interest has expanded and the traditional methods for gene fu- tion analysis cannot achieve the throughput necessary for large-scale exploration. Although computational tools such as BLAST remain a good point of departure, it is often the case that a gene that appears interesting in a hi- throughput experiment shows no obvious similarity to a gene of known fu- tion. In addition, when BLAST does find a similar gene, the process has often only begun.…

Codice articolo 3643358/2

Titolo
Gene Function Analysis
Autore
Unbekannt
Editore
Humana Press
Anno di pubblicazione
2007
Condizione
Sehr gut
Rilegatura
Rilegato
Lingua
inglese
ISBN 10
1588297349
ISBN 13
9781588297341
Cataloghi dei venditori
Bücher

Buchpark

Trebbin, Germania

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Venditore AbeBooks dal 30 settembre 2021

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