Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols

Simon J. Elsässer

ISBN 10: 1071632507 / ISBN 13: 9781071632505

Editore: Springer US, 2023

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Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Codice articolo 9781071632505

Dati bibliografici

• Titolo: Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols
• Editore: Springer US
• Data di pubblicazione: 2023
• Lingua: Inglese
• ISBN 10: 1071632507
• ISBN 13: 9781071632505
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 260 L x 183 A x 22 P (millimetri) 260 millimetri di larghezza per 183 millimetri di altezza per 22 millimetri di profondità
• Peso: 765 grammi

Descrizione articolo

Riassunto

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.