Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols

Simon J. Elsässer

ISBN 10: 1071632531 / ISBN 13: 9781071632536

Editore: Springer New York, 2023

Riguardo questo articolo

Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Codice articolo 9781071632536

Dati bibliografici

• Titolo: Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols
• Editore: Springer New York
• Data di pubblicazione: 2023
• Lingua: Inglese
• ISBN 10: 1071632531
• ISBN 13: 9781071632536
• Rilegatura: Taschenbuch
• Condizione: Neu
• Dimensioni: 254 L x 178 A x 17 P (millimetri) 254 millimetri di larghezza per 178 millimetri di altezza per 17 millimetri di profondità
• Peso: 569 grammi

Descrizione articolo

Riassunto

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.