The chemical and physical principles of fixation, staining, and histochemistry in one volume! Now in its fourth edition,Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations.
Reviews:
"John Kiernan has published extensively on histochemical methods since his first scientific paper in 1964. His textbook encompasses his wide personal experience and interest in dyes, staining and histochemical methodologies... I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source."
Raymond Coleman, Acta Histochemica, April 2010'This book would serve as an excellent introduction for undergraduate and PhD students and will easily guide researchers and technicians in the comprehension of new techniques. The author emphasizes the importance of acquiring laboratory skills under the guidance of more experienced colleagues, which of course is true, but this book provides a wonderful introduction to the new researcher and provides alternatives to the more experienced colleague . This book is a must have for any laboratory using histological techniques. It is an excellent teaching resource for anyone new to the field and gives ideas to the more experienced histologist. This book is not for the bookshelf it should live on your laboratory bench.' --Journal of Anatomy
'I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source. It is not a book to be read from start to finish, but should be consulted by researchers and in particular students before undertaking histochemical staining procedures. The book is very reasonably priced and to be objective, the purchase price is less than the cost of one or two antibodies or most laboratory chemicals, which, unlike a book, have a very limited life.' --Acta Histochemica
'The book is written in a succinct manner and is very readable despite being a technical manual. Numerous diagrams on chemical structures and reactions with excellent legends also make this book very user-friendly. Besides providing the usual cookbook-type recipes for the various staining methods, the author also carefully explains the theory and biochemistry behind the methodology. This information, a valuable asset in troubleshooting, would be very helpful to students, histotechnologists, and even pathologists and researchers...the concise and well written text makes this book a very handy manual for all laboratories involved in histology and histochemistry.' --Journal of Histotechnology
'I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source. It is not a book to be read from start to finish, but should be consulted by researchers and in particular students before undertaking histochemical staining procedures. The book is very reasonably priced and to be objective, the purchase price is less than the cost of one or two antibodies or most laboratory chemicals, which, unlike a book, have a very limited life.' --Acta Histochemica
'The book is written in a succinct manner and is very readable despite being a technical manual. Numerous diagrams on chemical structures and reactions with excellent legends also make this book very user-friendly. Besides providing the usual cookbook-type recipes for the various staining methods, the author also carefully explains the theory and biochemistry behind the methodology. This information, a valuable asset in troubleshooting, would be very helpful to students, histotechnologists, and even pathologists and researchers...the concise and well written text makes this book a very handy manual for all laboratories involved in histology and histochemistry.' --Journal of Histotechnology
