Hydra: Research Methods

Lingua: inglese

Editore: Springer, 2013

1475705980 / 9781475705980

Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH

Venditore con 5 stelle

Venditore AbeBooks dal 14 agosto 2006

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Druck auf Anfrage Neuware - Printed after ordering - InhaltsangabeTerminology for Morphology and Cell Types.- Terminology for Morphology and Cell Types.- Culture and Handling.- Collecting Hydra.- Identifying Hydra Species.- Water, Culture Solutions, and Buffers.- Visual Monitoring of pH in Solutions with Phenol Red.- Hatching Brine Shrimp Larvae Axenically and/or in a Range of Quantities.- Determining Growth Rates of Groups of Hydra and Budding Rates of Individual Hydra.- Culturing Large Numbers of Hydra.- Turbidimetric and Pipetimetric Measurements of Number of Hydra.- Culturing Hydra of the Same Species but of Different Sizes.- Culture of Sexually Differentiated Hydra.- Preparing Axenic Hydra.- Histology.- Preparing Hydra for Transmission Electron Microscopy.- Preparing Hydra for Scanning Electron Microscopy.- Preparing Hydra for Freeze-Fracture and Freeze-Etching.- Whole Mounts for Light Microscopy.- Preparing Histological Sections for Light Microscopy.- Vital Staining: Fluorescent and Immunofluorescent, and Review of Nonfluorescent Dyes.- Macrophotography.- Macrophotography.- Quantitative Cytology.- Dissociating Hydra Tissue into Single Cells by the Maceration Technique.- Cell Cycle Analysis of Hydra Cells.- Mitotic Index.- Measurement of the Numbers of Nematoblasts, Nematocytes, and Nematocysts.- Marking Epithelial Cells in Living Hydra with India Ink.- Techniques Using Isotopes.- Incorporating [3H]Thymidine into Hydra by Microinjection.- Labeling with Gaseous 14CO2 or by Feeding on Radioactive Tissues.- Fractionating Small Amounts of Radioactive Tissue.- Rapid Whole-Mount Radioautography.- Isolating Hydra Mutants by Sexual Inbreeding.- Isolating Hydra Mutants by Sexual Inbreeding.- Manipulating Tissue Organization.- Grafting: A Rapid Method for Transplanting Tissue.- Quantitative Interpretation of Transplantation Phenomena.- Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells.- Culturing Interstitial Stem Cells in Hydra Aggregates.- Separating Viable Tissue Layers.- Preparing Ectoderm/Endoderm Chimeras.- Manipulating Cellular Composition in Vivo.- Eliminating All Nonepithelial Cells Using Colchicine.- Culturing Epithelial Hydra.- Reducing Populations of Interstitial Cells and Nematoblasts with Hydroxyurea.- Preparing Hydra viridis with Nerve Cells and No Interstitial Cells, or with Neither of These Cell Types.- Eliminating Interstitial Cells with Nitrogen Mustard.- Altering Cell Population Levels by Gamma Irradiation.- Reducing Number of Nematocytes in the Tentacles.- Assay and Isolation of Substances Controlling Morphogenesis in Hydra.- Assay and Isolation of Substances Controlling Morphogenesis in Hydra.- Isolation and/or Properties of Acellular Mesoglea and Nematocysts.- Isolating Mesolamellae.- Isolating Undischarged and Discharged Nematocysts from Acontiate Sea Anemones.- Dissolving the Nematocyst Capsule Wall and Identifying its Protein Component(s).- Purifying an Inhibitor of Succinoxidase Activity from Hydra littoralis.- Assays for Activities of Nematocyst Venoms and Their Components.- Analytical Procedures.- Special Techniques for Weighing Microgram Quantities of Tissue and Assaying Them for Enzyme Activities.- Extracting and Characterizing Hydra RNA: Modifications to Allow Extraction of Undegraded Material in the Presence of High Levels of Degradative Enzymes.- Colorimetric Analysis for Protein of Hydra.- Determining Respiration and Oxygen Evolution of Green Hydra with the Rank Brothers Oxygen Electrode.- Symbiotic Relationships.- Isolating Endosymbiotic Algae from Hydra viridis.- Preparing Aposymbiotic Hydra.- Introducing Symbiotic Algae into Aposymbiotic Hydra.- Measuring Number of Algal Symbionts in Hydra viridis.- Measuring in Vivo Translocation of Reduced Organic Carbon Compounds from Endosymbiotic Algae to Hydra.- Spectrophotometric Assay for Maltose.- Methods for Epizootilogical Research with Hydras.- Experimental Methods for Epizootilogical Research with Hydras.- Electroph.…

Codice articolo 9781475705980

Titolo
Hydra: Research Methods
Autore
Howard Lenhoff
Editore
Springer
Anno di pubblicazione
2013
Condizione
Neu
Rilegatura
Taschenbuch
Lingua
inglese
ISBN 10
1475705980
ISBN 13
9781475705980
Peso dell'articolo
1274 grammi
Dimensioni
297x210x27 mm

AHA-BUCH GmbH

Einbeck, Germania

Venditore con 5 stelle

Venditore AbeBooks dal 14 agosto 2006

Tariffe di spedizione da Germania a U.S.A.

ArticoloDa 7 a 10 giorni lavorativiDa 5 a 7 giorni lavorativi
Primo articoloEUR 43,92EUR 60,29
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