Lab Manual and Workbook in Microbiology: Applications to Patient Care
Lingua: inglese
Editore: McGraw-Hill Education, 2013
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- Usato

Da: 3rd St. Books, Lees Summit, MO, U.S.A.3rd St. Books
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Codice articolo 061081
- Titolo
- Lab Manual and Workbook in Microbiology: Applications to Patient Care
- Autore
- Morello, Josephine; Granato, Paul; Morton, Verna
- Editore
- McGraw-Hill Education
- Anno di pubblicazione
- 2013
- Condizione
- Acceptable
- Rilegatura
- Spiral Bound
- Lingua
- inglese
- ISBN 10
- 0073402397
- ISBN 13
- 9780073402390
The authors have emphasized the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. The exercises illustrate as simply as possible the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilization. Attention is given to the role of the health professional in regard to appropriate collection of clinical specimens and the applications of aseptic and disinfectant techniques as they relate to patient care.
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Contenuti
1. The Microscope
2. Handling and Examining Cultures
3. Simple Stains, Hanging Drop, and Wet-Mount Preparations
Experiment 3.1 Preparing a Simple Stain
Experiment 3.2 Preparing a Hanging Drop
Experiment 3.3 Preparing a Wet Mount
5. Acid-Fast Stain
6. Special Stains
Experiment 6.1 Staining Bacterial Endospores (Schaeffer-Fulton Method)
Experiment 6.2 Flagella and Capsule Stains
8. Streaking Technique to Obtain Pure Cultures
9. Pour Plate and Subculture Techniques
Experiment 9.1 Pour-Plate Technique
Experiment 9.2 Subculture Technique (Picking Isolated Colonies for Pure Cultures)
PART TWO Destruction of Microorganisms
Physical Antimicrobial Agents
Chemical Antimicrobial Agents
Exercises
Experiment 10.1 Moist Heat
Experiment 10.2 Dry Heat
Experiment 10.3 Incineration
12. Disinfectants
Case Study: A Lesson Learned—Follow Proper Procedures for the Disinfection of Equipment
13. Antimicrobial Agent Susceptibility Testing and ResistanceExperiment 13.1 Disk Agar Diffusion Method
Experiment 13.2 Broth Dilution Method: Determining Minimum Inhibitory Concentration (MIC)
PART THREE Diagnostic Microbiology in Action
General Considerations
Microbiology at the Bedside
Collection and Transport of Clinical Specimens for Culture
Precautions for Handling Specimens and Cultures
Normal Flora of the Body
Principles of Diagnostic Microbiology
Exercises
15. Some Metabolic Activities of Bacteria
Experiment 15.1 Simple Carbohydrate Fermentations
Experiment 15.2 Starch Hydrolysis
Experiment 15.3 The Indole Test
Experiment 15.4 Use of Kligler Iron Agar
Experiment 16.1 The Activity of Urease
Experiment 16.2 The Activity of Catalase
Experiment 16.3 The Activity of Oxidase
Experiment 16.4 The Activity of Deoxyribonuclease (DNase)
Experiment 16.5 The Activity of a Deaminase
18. Staphylococci
Experiment 18.1 Isolation and Identification of Staphylococci
Experiment 18.2 Staphylococci in the Normal Flora Case Study: Community-Acquired Methicillin-Resistant Staphylococcus
Experiment 19.1 Identification of Group A Streptococci (Streptococcus pyogenes)
Case Study: A Case of Pharyngitis
Experiment 19.2 The CAMP Test for Group B Streptococci (Streptococcus agalactiae)
Experiment 19.3 Identification of Pneumococci (Streptococcus pneumoniae)
Case Study: A Case of Pneumonia with Meningitis
Experiment 19.4 Identification of Enterococci
Experiment 19.5 Streptococci in the Normal Flora
Experiment 20.1 Haemophilus
Experiment 20.2 Corynebacteria
Experiment 20.3 Bordetella
Case Study: A School-Associated Pertussis Outbreak
Experiment 21.1 Laboratory Diagnosis of a Sore Throat
Experiment 21.2 Laboratory Diagnosis of Bacterial Pneumonia
Experiment 21.3 Antimicrobial Susceptibility Test of an Isolate from a Clinical Specimen
Experiment 22.1 Identification of Pure Cultures of Enterobacteriaceae from the Normal Intestinal Flora
Experiment 22.2 Isolation Techniques for Enteric Pathogens Case Study: A Case of Diarrhea
Experiment 22.3 Identification Techniques for Enteric Pathogens
Experiment 22.4 Serological Identification of Enteric Organisms
Experiment 22.5 Techniques to Distinguish Nonfermentative Gram-Negative Bacilli from Enterobacteriaceae
Experiment 22.6 Rapid Methods for Bacterial Identification 23. Clinical Specimens from the Intestinal Tract
Experiment 23.1 Culturing a Fecal Sample
Experiment 23.2 Identification of an Unknown Enteric Organism
Experiment 23.3 Antimicrobial Susceptibility Test of an Enteric Organism
Experiment 24.1 Examination and Qualitative Culture of Voided Urine
Experiment 24.2 Quantitative Urine Culture
Case Study: A Case of Cystitis
Experiment 25.1 Neisseria
Experiment 25.2 Spirochetes
Case Study: A Case of a Patient with a Bull’s-Eye Skin Lesion
Case Study: Three Patients with Positive Blood Cultures
PART FOUR Microbial Pathogens Requiring Special Laboratory Techniques; Serodiagnosis of Infectious Disease; Principles and Practices of Infection Prevention Exercises
27. Anaerobic BacteriaCase Study: A Case of Diarrhea in a Hospitalized Patient
28. MycobacteriaExperiment 28.1 Microscopic Morphology of Mycobacterium tuberculosis
Experiment 28.2 Culturing a Sputum Specimen for Mycobacteria 254
Case Study: A Case of Pneumonia in a Patient with Chronic Alcoholism
29. Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions
30. Fungi:Yeasts and Molds
31. Protozoa and Animal Parasites
32. Serodiagnosis of Infectious Disease
33. Principles and Practices of Infection Prevention
Experiment 33.1 Nasal Surveillance Cultures for MRSA
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