This laboratory manual and workbook, now in its Eighth Edition, maintains its original emphasis on the basic principles of diagnostic microbiology for students preparing to enter the allied health professions. It remains oriented primarily toward meeting the interests and needs of those who will be directly involved in patient care and who wish to learn how microbiological principles should be applied in the practice of their professions.
1 Basic Techniques of Microbiology
1 Orientation to the Microbiology Laboratory
1 The Microscope
2 Handling and Examining Cultures
2 Microsopic Morphology of Microorganisms
3 Hanging-Drop and Wet-Mount Preparations
4 Simple Stains
3 Differential Stains
5 Gram Stain
6 Acid-Fast Stain
7 Special Stains
4 Cultivation of Mircroorganisms
8 Culture Media
9 Streaking Technique to Obtain Pure Cultures
10 Pour-Plate and Subculture Techniques
11 Culturing Microorganisms from the Environment
2 Destruction of Microorganisms
5 Physical Antimicrobial Agents
12 Moist and Dry Heat
13 The Autoclave
6 Chemical Antimicrobial Agents
14 Disinfectants
15 Antimicrobial Agent Susceptibility Testing and Resistance
3 Diagnostic Microbiology in Action
7 Principles of Diagnostic Microbiology: Culture of Clinical Specimens, Identifying Isolated Microorganisms, Antigen Detection and Nucleic Acid Assays
16 Primary Media for Isolation of Microorganisms
17 Some Metabolic Activities of Bacteria
18 Activities of Bacterial Enzymes
19 Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms
8 Microbiology of the Respiratory Tract
20 Staphylococci
21 Streptococci, Pneumococci, and Enterococci
22 Haemophilus, Corynebacteria, and Bordetella
23 Clinical Specimens from the Respiratory Tract
9 Microbiology of the Intestinal Tract
24 The Enterobacteriaceae
25 Clinical Specimens from the Intestinal Tract
10 Microbiology of the Urinary and Genital tracts
26 Urine Culture Techniques
27 Neisseria and Spirochetes
11 Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients' Antibodies
28 Anaerobic Bacteria
29 Mycobacteria
30 Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions
31 Fungi: Yeasts and Molds
32 Protozoa and Animal Parasites
33 Serological Identification of Patients' Antibodies
4 Applied (Sanitary) Microbiology
34 Bacteriologic Analysis of Water
35 Bacteriologic Analysis of Milk