Leptospira spp.: Methods and Protocols (Methods in Molecular Biology)

ISBN 10: 1071604619 / ISBN 13: 9781071604618

Editore: Humana, 2021

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1st ed. 2020 edition NO-PA16APR2015-KAP.

Codice articolo 26387310128

Dati bibliografici

• Titolo: Leptospira spp.: Methods and Protocols (Methods in Molecular Biology)
• Editore: Humana
• Data di pubblicazione: 2021
• Lingua: Inglese
• ISBN 10: 1071604619
• ISBN 13: 9781071604618
• Rilegatura: Brossura
• Condizione: New

Descrizione articolo

Riassunto

This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.