In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Recensione:

"...those who deal with liposome in their work, this book with detailed laboratory protocols and many notes will be very useful." -Pharmaceutical Research

"Volume 199 of the series Methods in Molecular Biology™, offers an excellent overview of protocols used for the study of liposomes in biology...the book portrays comprehensive reports of the study of the cellular fate of sphingolipids using fluoresrole lipids play in virus fusion, studies of functions of various membrane-associated proteins, specifically of phospholipid binding proteins in glycoprotein biosynthesis and contributions of therapeutical liposome applications." -Molecular Biotechnology

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