In Living Color: Protocols in Flow Cytometry and Cell Sorting (Springer Lab Manuals)

Rochelle A. Diamond, Susan Demaggio

ISBN 10: 3540651497 / ISBN 13: 9783540651499

Editore: Springer, 2000

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Codice articolo ERICA77335406514976

Dati bibliografici

• Titolo: In Living Color: Protocols in Flow Cytometry and Cell Sorting (Springer Lab Manuals)
• Editore: Springer
• Data di pubblicazione: 2000
• Lingua: Inglese
• ISBN 10: 3540651497
• ISBN 13: 9783540651499
• Rilegatura: Hardcover
• Condizione: Like New
• Tipo di libro: book

Descrizione articolo

Riassunto

A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.

Contenuti

Introduction.- History.- Getting started.- Sample preparation and cell surface staining.- Reporter genes and cell trackers.- Flow cytometry of nucleic acids.- Intracellular physiological and antibody probes.- Electronic cell sorting.- Core facilities.- Unlocking the spectrum of possibilities.- Appendix.