Matrix Metalloproteases: Methods and Protocols (Methods in Molecular Biology, Band 1579) : Methods and Protocols

Charles A. Galea

ISBN 10: 1493983210 / ISBN 13: 9781493983216

Editore: Springer New York, 2018

Riguardo questo articolo

Gebraucht - Sehr gut SG - Ungelesenes Mängelexemplar, gestempelt, mit leichten Lagerspuren - Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo , and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Codice articolo INF1000547867

Dati bibliografici

• Titolo: Matrix Metalloproteases: Methods and Protocols (Methods in Molecular Biology, Band 1579) : Methods and Protocols
• Editore: Springer New York
• Data di pubblicazione: 2018
• Lingua: Inglese
• ISBN 10: 1493983210
• ISBN 13: 9781493983216
• Rilegatura: Taschenbuch
• Condizione: Sehr gut
• Dimensioni: 254 L x 178 A x 17 P (millimetri) 254 millimetri di larghezza per 178 millimetri di altezza per 17 millimetri di profondità
• Peso: 619 grammi

Descrizione articolo

Riassunto

Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.