Memory B-Cells

Kim L. Good-Jacobson

ISBN 10: 1071639498 / ISBN 13: 9781071639498

Editore: Springer US Jul 2024, 2024

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Neuware -This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 264 pp. Englisch.

Codice articolo 9781071639498

Dati bibliografici

• Titolo: Memory B-Cells
• Editore: Springer US Jul 2024
• Data di pubblicazione: 2024
• Lingua: Inglese
• ISBN 10: 1071639498
• ISBN 13: 9781071639498
• Rilegatura: Buch
• Condizione: Neu
• Dimensioni: 260 L x 183 A x 20 P (millimetri) 260 millimetri di larghezza per 183 millimetri di altezza per 20 millimetri di profondità
• Peso: 700 grammi

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Riassunto

This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.