Metabolic Flux Analysis in Eukaryotic Cells | Methods and Protocols

Deepak Nagrath

ISBN 10: 107160161X / ISBN 13: 9781071601617

Editore: Springer US, 2021

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Metabolic Flux Analysis in Eukaryotic Cells | Methods and Protocols | Deepak Nagrath | Taschenbuch | xiii | Englisch | 2021 | Springer US | EAN 9781071601617 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

Codice articolo 119467213

Dati bibliografici

• Titolo: Metabolic Flux Analysis in Eukaryotic Cells | Methods and Protocols
• Editore: Springer US
• Data di pubblicazione: 2021
• Lingua: Inglese
• ISBN 10: 107160161X
• ISBN 13: 9781071601617
• Rilegatura: Taschenbuch
• Condizione: Neu
• Dimensioni: 254 L x 178 A x 21 P (millimetri) 254 millimetri di larghezza per 178 millimetri di altezza per 21 millimetri di profondità
• Peso: 721 grammi
• Cataloghi dei venditori:
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Descrizione articolo

Riassunto

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.