Metabolic Flux Analysis : Methods and Protocols

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ISBN 10: 1493911694 / ISBN 13: 9781493911691

Editore: Springer New York, 2014

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Zustand: Sehr gut | Sprache: Englisch | Produktart: Bücher.

Codice articolo 24738440/12

Dati bibliografici

• Titolo: Metabolic Flux Analysis : Methods and Protocols
• Editore: Springer New York
• Data di pubblicazione: 2014
• Lingua: Inglese
• ISBN 10: 1493911694
• ISBN 13: 9781493911691
• Rilegatura: Rilegato
• Condizione: Sehr gut
• Cataloghi dei venditori:
  • Bücher

Descrizione articolo

Riassunto

Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from ¹³C experiments and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.