Methods for General and Molecular Microbiology

A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods.

• Provides a comprehensive compendium of methods used in general and molecular microbiology.
• Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis.
• Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions.
• Presents methods for bacteria, archaea, and for the first time a section on mycology.
• Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

This title is published by the American Society for Microbiology Press and distributed by Taylor and Francis in rest of world territories.

Table of Contents I. Morphology and Ultrastructure Introduction to Morphology and Ultrastructure, T. J. Beveridge, Editor 1. Light Microscopy, R. G. E. Murray and Carl F. Robinow 2. Sampling and Staining for Light Microscopy, Terry J. Beveridge, J. R. Lawrence, and Robert G. E. Murray 3. Laser Scanning Microscopy, John R. Lawrence and T. R. Neu 4. Electron Microscopy, Terry J. Beveridge, Dianne Moyles, and Bob Harris 5. Computational Image Analysis and Reconstruction from Transmission Electron Micrographs, George Harauz 6. Atomic Force Microscopy, Yves F. Dufrêne 7. Cell Fractionation, Susan F. Koval and G. Dennis Sprott 8. Antigen-Antibody Reactions, Lucy M. Mutharia and Joseph S. Lam II. Growth Introduction to Growth, John A. Breznak, Editor 9. Growth Measurement, Arthur L. Koch 10. Nutrition and Media, David Emerson, Jane Tang, Rosali J. Cote, and Robert L. Gherna 11. Enrichment and Isolation, Andreas Teske, Herbert Cypionka, John G. Holt, and Noel R. Krieg 12. Culture Techniques, Syed A. Hashsham 13. Energetics, Stoichiometry, and Kinetics of Microbial Growth, Syed A. Hashsham and Sam W. Bauschke 14. Physicochemical Factors in Growth, John A. Breznak and Ralph N. Costilow 15. Phenotypic Characterization and the Principles of Comparative Systematics, Brian J. Tindall, Johannes Sikorski, Robert A. Smibert, and Noel R. Krieg 16. General Methods to Investigate Microbial Symbioses, Todd A. Ciche and Shana K. Goffredi III. Metabolism Introduction to Metabolism, C. A. Reddy, Editor 17. Physical Analysis and Purification Methods, Scott B. Mulrooney, Willis A. Wood, and J. Robert Paterek 18. Chemical Analysis, Lacy Daniels, Richard S. Hanson, and Jane A. Phillips 19. Enzymatic Activity, Robert P. Hausinger and Allen T. Phillips 20. Permeability and Transport, Robert E. Marquis 21. Bacterial Respiration, Robert P. Gunsalus, Gary Cecchini, and Imke Schröder 22. Carbohydrate Fermentations, R. Meganathan, Yamini Ranganathan, and C. A. Reddy 23. Metabolism of Aromatic Compounds, Jerome J. Kukor, Boris Wawrik, and Gerben J. Zylstra 24. Cellulases, Hemicellulases, and Pectinases, Michael E. Himmel, John O. Baker, William S. Adney, and Stephen R. Decker 25. Lignin and Lignin-Modifying Enzymes, Carlos G. Dosoretz and C. A. Reddy IV. Molecular Genetics Introduction to Molecular Genetics, C. A. Reddy, Editor L. R. Snyder, Co-Editor 26. Similarity Analysis of DNAs, John L. Johnson and William B. Whitman 27. Nucleic Acid Analysis, William Hendrickson and Donald Walthers 28. Measuring Spontaneous Mutation, Patricia L. Foster 29. Transposon Mutagenesis, Silvia Rossbach and Frans J. de Bruijn 30. Plasmids, Marcelo E. Tolmasky, Luis A. Actis, Timothy J. Welch, and Jorge H. Crosa 31. Gene Transfer in Gram-Negative Bacteria, Joseph E. Peters 32. Gene Exchange in Gram-Positive Bacteria, Christopher J. Kristich, Christine E. Salomon, and Gary M. Dunny 33. Genetics of Archaea, Kevin R. Sowers, Paul H. Blum, and Shiladitya DasSarma 34. Genetic Manipulations Using Phages, Graham F. Hatfull, Debbie Jacobs-Sera, Michelle H. Larsen, and William R. Jacobs, Jr. V. Community and Genomic Analysis Introduction to Community and Genomic Analysis, Thomas M. Schmidt, Editor 35. Characterization of Bacterial Genome Sequences by Similarity Searching, William R. Pearson 36. Reconstructing and Interpreting Evolutionary Relationships, Christophe J. Douady and Camilla L. Nesbø 37. Microbial Nucleotide Fingerprints in Nature, David M. Karl 38. Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations, Edward F. DeLong 39. Single Cell Identification by Fluorescence In Situ Hybridization, Bernhard M. Fuchs, Jakob Pernthaler, and Rudolf Amann 40. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes, Daniel H. Buckley and Thomas M. Schmidt 41. Analysis of Microbial Communities with Denaturing Gradient Gel Electrophoresis and Terminal Restriction Fragment Length Polymorphism, Terence L. Marsh and Cindy H. Nakatsu VI. Mycology Introduction to Mycology, George A. Marzluf, Editor 42. Methods for Studying Terrestrial Fungal Ecology and Diversity, R. G. Thorn, J. Scott, and M. A. LaChance 43. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, George A. Marzluf 44. Microbiological and Genetic Methods for Filamentous Fungi, Rowland H. Davis and A. John Clutterbuck 45. Principles and Practice of DNA Microarray Technology, Krishnamurthy Natarajan, Matthew J. Marton, and Alan G. Hinnebusch APPENDICES: 46. Laboratory Safety, W. Emmett Barkley and C. A. Mickelson 47. Culture Preservation, Robert L. Gherna and C. A. Reddy