Methods of Protein Microcharacterization

Lingua: inglese

Editore: Humana Press, Humana Press Jul 1986, 1986

0896030903 / 9780896030909

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This item is printed on demand - Print on Demand Titel. Neuware -Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a 'micro' sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the 'gas'-phase sequencer of Hewick et al. (9) in 1981.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 480 pp. Englisch.…

Codice articolo 9780896030909

Titolo
Methods of Protein Microcharacterization
Autore
John E. Shively
Editore
Humana Press, Humana Press Jul 1986
Anno di pubblicazione
1986
Condizione
Neu
Rilegatura
Buch
Lingua
inglese
ISBN 10
0896030903
ISBN 13
9780896030909
Peso dell'articolo
842 grammi
Dimensioni
235x157x31 mm

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