MicroRNA Profiling : Methods and Protocols

Sweta Rani

ISBN 10: 1071628259 / ISBN 13: 9781071628256

Editore: Springer US, Springer US, 2023

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Druck auf Anfrage Neuware - Printed after ordering - This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,MicroRNA Profiling: Methods and Protocols, Second Editionaims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Codice articolo 9781071628256

Dati bibliografici

• Titolo: MicroRNA Profiling : Methods and Protocols
• Editore: Springer US, Springer US
• Data di pubblicazione: 2023
• Lingua: Inglese
• ISBN 10: 1071628259
• ISBN 13: 9781071628256
• Rilegatura: Taschenbuch
• Edizione: seconda edizione
• Condizione: Neu
• Dimensioni: 254 L x 178 A x 15 P (millimetri) 254 millimetri di larghezza per 178 millimetri di altezza per 15 millimetri di profondità
• Peso: 511 grammi

Descrizione articolo

Riassunto

This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.