Microarrays in Inflammation (Progress in Inflammation Research)

Gerstmayer Bernhard Bosio Andreas

ISBN 10: 376438333X / ISBN 13: 9783764383336

Editore: Springer, 2008

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Dati bibliografici

• Titolo: Microarrays in Inflammation (Progress in Inflammation Research)
• Editore: Springer
• Data di pubblicazione: 2008
• Lingua: Inglese
• ISBN 10: 376438333X
• ISBN 13: 9783764383336
• Rilegatura: Rilegato
• Condizione: New

Descrizione articolo

Riassunto

This book summarizes the most advanced technical aspects covering all steps for a thorough application of microarrays to inflammation topics – from sample generation to data analysis. In addition selected examples of successful applications of microarrays in major fields of inflammation research are presented. The book will help a researcher or clinician to plan, perform and analyze or to critically review microarray experiments related to inflammation research.

Contenuti

Microarray and inflammation: An introduction.- Microarray and inflammation: An introduction.- Methods and protocols for the generation of gene expression profiles in inflammation research.- Whole blood.- Separation of whole blood cells and its impact on gene expression.- Skin and skin models.- Adipose tissue.- Samples isolated by flow cytometry.- Magnetic cell sorting.- T7 based amplification protocols.- Amplification of cDNA from single or rare cells by global PCR (exponential amplification).- Selected applications of microarrays in inflammation research.- Gene expression patterns in asthma.- Gene expression profiling in neurological and neuroinflammatory diseases.- Aspects of gene expression in B cell lymphomas.- The genes behind rheumatology.- miRNA patterns in hematopoietic malignancies.- Keep it simple: microarray cross-platform comparison without statistics.- Microarrays in drug development: regulatory perspective.- Outlook.- Complementary microarray technologies.