Microfluidic Technologies for Single-Cell Analysis : Creating uniform cellular microenvironments for fast timescale measurements

Dino Di Carlo

ISBN 10: 3639139518 / ISBN 13: 9783639139518

Editore: VDM Verlag Dr. Müller, 2009

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nach der Bestellung gedruckt Neuware - Printed after ordering - Analysis of single cells allows a completeunderstanding of the heterogeneity that is present incell behavior and function. Current single-cellanalysis methods provide high-throughput informationabout labeled biomolecules within cells, but oftencannot follow the dynamic processes occurring insignaling pathways. New microfluidic methods haveseparately allowed assays of fast timescale responsesor creation of uniform environments to study cellbehavior in a more quantitative manner. This bookfocuses on microfluidic hydrodynamic trappingtechniques that aid in both fast timescalemeasurements and uniform environmental control in asingle platform. Example applications for fasttimescale analysis of pore-forming toxin insertioninto membranes and assays of single-cell enzymecontent will be presented. The book would be ofinterest to analytical chemists, engineers, andbiologists developing platforms to study single-cellbehavior. Also, biologists looking for tools tostudy cells of interest with statistical accuracywould find the book most helpful.

Codice articolo 9783639139518

Dati bibliografici

• Titolo: Microfluidic Technologies for Single-Cell Analysis : Creating uniform cellular microenvironments for fast timescale measurements
• Editore: VDM Verlag Dr. Müller
• Data di pubblicazione: 2009
• Lingua: Inglese
• ISBN 10: 3639139518
• ISBN 13: 9783639139518
• Rilegatura: Taschenbuch
• Condizione: Neu
• Peso: 168 grammi

Descrizione articolo

Riassunto

Analysis of single cells allows a complete understanding of the heterogeneity that is present in cell behavior and function. Current single-cell analysis methods provide high-throughput information about labeled biomolecules within cells, but often cannot follow the dynamic processes occurring in signaling pathways. New microfluidic methods have separately allowed assays of fast timescale responses or creation of uniform environments to study cell behavior in a more quantitative manner. This book focuses on microfluidic hydrodynamic trapping techniques that aid in both fast timescale measurements and uniform environmental control in a single platform. Example applications for fast timescale analysis of pore-forming toxin insertion into membranes and assays of single-cell enzyme content will be presented. The book would be of interest to analytical chemists, engineers, and biologists developing platforms to study single-cell behavior. Also, biologists looking for tools to study cells of interest with statistical accuracy would find the book most helpful.

L'autore

Dino Di Carlo is an Assistant Professor of Bioengineering at UCLA. He received his B.S. and Ph.D. in Bioengineering from UC Berkeley and San Francisco. He then conducted postdoctoral studies at Harvard Medical School and MGH. His research focuses on miniaturization technologies for advanced diagnostic and automated single cell analysis systems.