Molecular Systematics

ISBN 10: 0878932828 / ISBN 13: 9780878932825

Editore: Sinauer Associates Inc, 1996

The book is clean and shows minor shelf ware, Codice articolo EBBV.0878932828.VG

Riassunto:

In this second edition, all of the chapters have been updated and extensively revised to include recent advances in techniques and methods of analysis, and a separate chapter on the polymerase chain has been added. Each chapter emphasizes the conceptual background needed to understand the subject, and then provides a guide to collecting or analyzing data. Included are chapters on sampling design, collection and storage of tissues (a guide to major tissue collections has been added), each of the major molecule techniques, interspecific and phylogenetic analysis, and a review of applications. The technique chapters cover principles, assumptions, applications, limitations, and basic protocols for isozyme electrophoresis, molecular cytogenetics, DNA hybridization, the polymerase chain reaction (PCR), DNA fragments (including microsatellites and RAPDs), restriction sites analysis, cloning and nucleic acid sequencing. The chapters on analysis have been expanded to include the many new developments in techniques for analyzing molecular data, including an expanded discussion of maximum likelihood methods, methods for assessing the results, comparative methods, and models of molecular evolution.

Contenuti: (in brief)
Molecular Systematics: Context and Controversies
I SAMPLING
Project Design
Collection and Storage of Tissues
II MOLECULAR TECHNIQUES
Proteins: Isozyme Electrophoresis
Chromosomes: Molecular Cytogenetics
Nucleic Acids I: DNA-DNA Hybridization
Nucleic Acids II: The Polymerase Chain Reaction
Nucleic Acids III: Analysis of Fragments and Restriction Sites
Nucleic Acids IV: Sequencing and Cloning
III ANALYSIS
Intraspecific Differentiation
Phylogenetic Inference
Applications of Molecular Systematics: The State of the Field and a Look to the Future

Dati bibliografici

Titolo: Molecular Systematics
Casa editrice: Sinauer Associates Inc
Data di pubblicazione: 1996
Legatura: Brossura
Condizione: very_good
Edizione: seconda edizione