Nucleic Acid Structure: An Introduction

Guschlbauer, W.

ISBN 10: 0387901418 / ISBN 13: 9780387901411

Editore: Springer 1976-05, 1976

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Codice articolo 6666-IUK-9780387901411

Dati bibliografici

• Titolo: Nucleic Acid Structure: An Introduction
• Editore: Springer 1976-05
• Data di pubblicazione: 1976
• Lingua: Inglese
• ISBN 10: 0387901418
• ISBN 13: 9780387901411
• Rilegatura: PF
• Condizione: New

Descrizione articolo

Riassunto

Teaching a course on nucleic acid structure is a hazardous undertaking, especially if one has no continuous teaching obligations. Everything changed when, in the spring of 1973, I re­ ceived an invitation to teach such a course, under the UNESCO-OAS-Molecular Biology Program at the Universi­ dad de Chile in Santiago during October 1973.

Contenuti

1 Introduction.- 1.1 History.- 1.2 Cellular localization of nucleic acids.- 2 Methods and Techniques.- 2.1 Absorption and optical activity (ORD and CD).- 2.2 Nuclear magnetic resonance (NMR).- 2.3 Ultracentrifugation.- 2.4 X-ray fiber diffraction.- 3 Chemistry and Enzymology of Nucleic Acids.- 3.1 Chemistry of nucleic acids and their constituents.- 3.2 Chemical reactions of nucleic acids and their constituents.- 3.3 Isolation of nucleic acids.- 3.4 Degradation and determination of nucleic acids.- 4 Structure and Function of DNA.- 4.1 The double helix of Watson and Crick.- 4.2 Experimental tests of the Watson-Crick hypothesis.- 4.3 The role of DNA in the cellular machinery—the central dogma.- 5 Physical Chemistry of DNA—The Problems of DNA Research.- 5.1 Established facts.- 5.2 Facts that may require amendments of details of the Watson–Crick theory.- 5.3 What do we require from biologically operative DNA?.- 5.4 What do we still not know?.- 6 Model Systems for Nucleic Acids.- 6.1 Polynucleotides.- 6.2 Oligonucleotides.- 6.3 Association of monomers in solutions and crystals.- 7 Errors and Mutations.- 7.1 Chemical mutagenesis.- 7.2 Base analogues.- 7.3 Antibiotics, pigments, dyes.- 7.4 Mutagenesis by radiation (UV, X-rays).- 8 The Structure of Ribonucleic Acids.- 8.1 Protein biosynthesis.- 8.2 Transfer RNA.- 8.3 Ribosome structure and rRNA.- 8.4 Viral RNA and virus structure.- 9 Nucleic Acid—Protein Interactions.- 9.1 Polyamines.- 9.2 Polypeptides and protamines.- 9.3 Histones and chromatin.- In the Guise of an Epilogue.- Appendix: Abbreviations and Symbols for Nucleic Acids, Polynucleotides, and Their Constituents.- Suggested Bibliography for Advanced Reading.