PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (Methods in Molecular Biology)

ISBN 10: 0896033430 / ISBN 13: 9780896033436

Editore: Humana Pr Inc, 1997

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Riassunto:

Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.

Recensione: This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis.-Biochemical Education

Dati bibliografici

Titolo: PCR Cloning Protocols: From Molecular ...
Casa editrice: Humana Pr Inc
Data di pubblicazione: 1997
Legatura: spiral_bound
Condizione: Good