PCR Sequencing Protocols (Methods in Molecular Biology)

Rapley, Ralph (Editor)

ISBN 10: 1489940383 / ISBN 13: 9781489940384

Editore: Humana Press, 2013

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236 pages. 9.10x6.00x0.60 inches. In Stock. Codice articolo x-1489940383

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Titolo: PCR Sequencing Protocols (Methods in ...

Casa editrice: Humana Press

Data di pubblicazione: 2013

Legatura: Paperback

Condizione: Brand New

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Riassunto:

Advances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Recensione:

...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. -FEBS Letters

"The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols....I highly recommend this most comprehensive volume for all molecular biologists."- SMG Quarterly