Parameter estimation of Michaelis-Menten kinetics: Comparison between experimental design methodologies and equidistant sampling using genetic algorithms

Ataíde, Filipe, Hitzmann, Bernd

ISBN 10: 3639253345 / ISBN 13: 9783639253344

Editore: VDM Verlag Dr. Müller, 2010

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Codice articolo ERICA78736392533456

Dati bibliografici

• Titolo: Parameter estimation of Michaelis-Menten kinetics: Comparison between experimental design methodologies and equidistant sampling using genetic algorithms
• Editore: VDM Verlag Dr. Müller
• Data di pubblicazione: 2010
• Lingua: Inglese
• ISBN 10: 3639253345
• ISBN 13: 9783639253344
• Rilegatura: Paperback
• Condizione: Like New
• Tipo di libro: book

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Riassunto

The main objective of this work was to investigate the application of experimental design techniques for the identification of Michaelis-Menten kinetic parameters. More specifically, this study attempts to elucidate the relative advantages/disadvantages of employing complex experimental design techniques in relation to equidistant sampling when applied to different reactor operation modes. All studies were supported by simulation data of a generic enzymatic process that obeys to the Michaelis-Menten kinetic equation. Different aspects were investigated, such as the influence of different reactor operation modes (batch, fed-batch with pulsewise feeding and fed- batch with continuous feeding) and the experimental design optimality criteria on the effectiveness of kinetic parameters identification.