Pcr in Bioanalysis. Questo articolo non è disponibile.Pcr in Bioanalysis

The invention of the polymerase chain reaction (PCR) eventually earned Kary B. Mullis half of the 1993 Nobel Prize for Chemistry (1–4). However, for several years, issues of quality control and reproducibility interfered with attempts at commercial or clinical application of PCR. More recently, persistent work and numerous methodological inno- tions and refinements have resulted in the establishment of PCR as a routine, sensitive, and specific detection method in hospital and agricultural labora- ries. This transformation of PCR from an experimental research technique to an established bioassay tool formed the impetus behind PCR in Bioanalysis. PCR has proven particularly valuable in clinical microbiology and the diagnosis of infectious diseases in humans and animals. This large and diverse group of applications is reviewed in Chapter 1 by Gorm Lisby. S- cific organisms now detectable by PCR include hepatitis C virus (protocols presented in Chapter 14), Mycobacterium tuberculosis (Chapter 18), Chla- dia and Trichomonas species (Chapter 20), Toxoplasma gondii (Chapter 17), Legionella species (Chapter 15), enterotoxigenic Escherichia coli (Chapters 9 and 10), HIV-1 subspecies (Chapter 8), bovine immunodeficiency-like virus (Chapter 6), rodent parvoviruses (Chapter 2), Ross River virus (Chapter 12), and porcine reproductive and respiratory syndrome virus (Chapter 7).

Application of Nucleic Acid Amplification in Clinical Microbiology Gorm Lisby Detection of Rodent Parvoviruses by PCR David G. Besselsen The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity Darryl Shibata Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using Fluorescence PCR and an Automated Sequencer Mei Wang and Maurice Godfrey. Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR Jay K. Kolls and Jianming Xie PCR Diagnosis of the Bovine Immunodeficiency-Like Virus David L. Suarez and Cecelia A. Whetstone PCR Analysis for the Identification of Porcine Reproductive and Respiratory Syndrome Virus in Boar Semen Jane Christopher-Hennings and Eric A. Nelson Quantitative and Discriminative Detection of Individual HIV-mRNA Subspecies by an RNase Mapping Assay Catherine Mary, Hideo Akaoka, and Bernard Verrier Detection of the Heat-Labile Toxin Coding Gene (LT-Gene) of Enterotoxigenic Escherichia coli Séamus Fanning, Deirdre O'Meara, Lesley Cotter, John O'Mullane, and Bartley Cryan Motif-Dependent Polymerase Chain Reaction (PCR): DNA Fingerprinting Entererotoxigenic Escherichia coli Séamus Fanning, Deirdre O'Meara, Lesley Cotter, Paddy Greer, and Bartley Cryan In Vitro Transcription/Translation Analysis for the Identification of Translation-Terminating Mutations Michael C. Luce, Cameron G. Binnie, Lauren N. W. Kam-Morgan, and Matthew C. Cayouette A Single-Tube Nested RT-PCR for the Detection of Ross River Virus Loryn Sellner Application of PCR to Transgenic Plants Michael Wassenegger Detection of Hepatitis C Virus RNA by Semiquantitative Reverse-Transcription PCR Anderson S. Gaweco and David H. Van Thiel Detection of Legionella Species in Bronchial Fluid by PCR GormLisby Quantitative Measurement of mRNA Expression by Competitive RT-PCR Joe O'Connell, Triona Goode, and Fergus Shanahan PCR Detection of Toxoplasma gondii in Human Fetal Tissues Tamás Tóth, István Sziller, and Zoltán Papp Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICORTM Mycobacterium tuberculosis PCR Test Richard F. D'Amato and Albert Miller The Use of Flow Cytometry and RT-PCR in the Detection of Circulating PSA-Positive Cells in Prostate Cancer Emma J. Fadlon and Freddie C. Hamdy Detection of Chlamydia trachomatis and Trichomonas vaginalis in the Vaginal Introitus, Posterior Vagina, and Endocervix by Polymerase Chain Reaction Jan Jeremias, Vera Tolbert, and Steven S. Witkin Detection and Isolation of Differentially Expressed Genes by PCR John M. Abraham Detection of Transgene Integrants and Homologous Recombinants in Mice by Polymerase Chain Reaction Kristin M. Abraham, Nancy S. Longo, and Judith A. Hewitt Direct Analysis for Familial Adenomatous Polyposis Mutations Steven M. Powell PCR Fingerprinting for Detection of Deleted or Amplified Sequences in Human Cancer Takashi Kohno and Jun Yokota Index