Pichia Protocols

James M. Cregg

ISBN 10: 1617375632 / ISBN 13: 9781617375637

Editore: SPRINGER NATURE Nov 2010, 2010

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This item is printed on demand - it takes 3-4 days longer - Neuware -This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition. 268 pp. Englisch. Codice articolo 9781617375637

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Titolo: Pichia Protocols

Casa editrice: SPRINGER NATURE Nov 2010

Data di pubblicazione: 2010

Legatura: Taschenbuch

Condizione: Neu

Edizione: seconda edizione

Descrizione articolo

Riassunto:

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).

Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

Dalla quarta di copertina:

<p>The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2^nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2^nd Edition, is an essential tool for researchers working with the Pichia Pastoris system</p>